Hcs lipidtox
WebThis probe is compatible with the HCS LipidTOX phospholipidosis detection reagents. HCS LipidTOX neutral lipid stains can also be used to monitor the formation and … WebSep 26, 2024 · For time-lapse imaging, live cells were treated with different agents and HCS LipidTOX red neutral lipid dye (Invitrogen, 1:200) was immediately added. ... LipidTOX staining confirmed the increase in lipid-droplet accumulation in these cells (Fig. 6E, F). Thus, BMP2 is a positive regulator of lipogenic differentiation in human IPF lung ...
Hcs lipidtox
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WebCitations & References The HCS LipidTOX™ Red phospholipidosis detection reagent was developed to characterize the potentially toxic side effects of compounds on lipid … WebHCS LipidTOX™ Deep Red neutral lipid stain was developed to characterize the potentially toxic effects of compounds on lipid metabolism in mammalian cell lines. The LipidTOX™ neutral lipid stain has an extremely high affinity for neutral lipid droplets and can be … HCS LipidTOX™ Green neutral lipid stain was developed to characterize the … HCS LipidTOX™ Deep Red neutral lipid stain was developed to characterize the … TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes
WebDec 17, 2016 · After 48 culture140 Glocket al. Vitro35 (2016) 139–148 mediumwas replaced freshmedium, including HCS LipidTOX Green phospholipidosis detection reagent (LipidTOX) (Invitrogen, Carlsbad, US) (1:1000), respectiveconcentration bina-ry combinations Samplescontaining LipidTOX respectivesolvent(s) (vehicle controls) were … WebThe LipidTOX™ neutral lipid stain has an extremely high affinity for neutral lipid droplets and can be detected by fluorescence microscopy or an HCS reader. This probe is …
WebJan 13, 2024 · Then, cells were stained with HCS LipidTOX Red neutral lipid stain (H34476, Thermo Fisher Scientific) for 30 min. Nuclei were stained with DAPI for 5 min. All slides were mounted with VECTASHIELD ... WebAug 8, 2013 · The assays were carried out with the HCS LipidTOX Phospholipidosis and Steatosis Detection Kit according to the manufacturer's instructions (Invitrogen). Xenograft Analysis. Nude mice (nu/nu, 6- to 8-week-old males) were injected subcutaneously with 1.5 × 10 5 A549 stable cells. The diameters of tumors were measured every 10 days.
WebFeb 7, 2024 · Images confirm that the mTOR inhibitor AZD8055 accentuates puncta formation revealed by the HCS LipidTox probe (Fig. 3c), suggesting that TMEM41B depletion and mTOR inhibition act synergistically to amplify lipid accumulation in the observed puncta. We note that the most significant increases in DUNN index were …
WebHcs Lipidtox Deep Red Neutral Lipid Stain, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - … converting serial numbers into dates in excelWebNov 9, 2024 · In a study using HCS LipidTOX Green phospholipidosis detection reagent for the identification of changes in rat and human macrophages after exposure to 0.1, 1, and 10 µM amiodarone, the following results were obtained after 24 h of exposure . Rat primary alveolar macrophages reacted differently from NR8383 and human U937 macrophages … converting sendmail to postfix part twoWebThermo Fisher hcs lipidtox green neutral lipid stain solution Hcs Lipidtox Green Neutral Lipid Stain Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more falls in portland jamaicaWebHCS LipidTOX Green Neutral Lipid is a fluorescent compound with an excitation peak at 498 nm and an emission peak at 507 nm. It can be excited using a 488 nm laser paired … converting shapefile to kmzWebDec 2, 2024 · Adipocyte lipid droplet staining was performed with HCS LipidTOX™ Deep Red Neutral Lipid Stain (Thermo Fisher, Waltham, MA, USA). After washing cells with PBS three times, the adipocytes were fixed with 4% formaldehyde and incubated for 30 min at room temperature. Next, the formaldehyde was removed, and the cells were gently … falls insurance agencyWebHCS LipidTOX Red solution from the manufacturer µl/10 ml PBS). Cells in MatTek dishes were stained after fixation by addition of 2 ml solution of dye in PBS. To reduce the effects of temperature variation on stain-ing efficiency, we incubated the fixed cells in staining solution for 30 min at 37C. They were then immedi- falls international market 2408 e.10th stWebHCS LipidTOX™ phospholipidosis detec-tion reagents can be easily detected with fluorescence microscopes or HCS readers equipped with standard filter sets (Figure … falls in seniors statistics