Cycloheximide stock
WebApr 12, 2024 · Make 2.0 mM working solution by diluting 0.5 μL stock per 100 μL culture medium. 14. Cycloheximide solution: Prepare stock with 1.0 mg/mL in PBS. Make a 10 μg/mL working solution in culture medium. 15. Oocyte activation medium (OAM): DMEM/F12 supplemented with 5.0% (v/v) FBS, 2.0 mM 6-DMAP, 10 μg/mL cycloheximide, and … WebCycloheximide Catalog No. A8244 Antibiotic,inhibitor of protein synthesis in eukaryotes Skip to the end of the images gallery Skip to the beginning of the images gallery Tel: +1 …
Cycloheximide stock
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WebApr 10, 2024 · The Saccharomyces cerevisiae Agp2 is a plasma membrane protein initially reported to be an uptake transporter for L-carnitine. Agp2 was later rediscovered, together with three additional proteins, Sky1, Ptk2, and Brp1, to be involved in the uptake of the polyamine analogue bleomycin-A5, an anticancer drug. Mutants lacking either Agp2, … WebCycloheximide (CHX) (Sigma Aldrich) is stored as a 500 μg/mL stock solution, solved in dimethylsulfoxide (DMSO) at − 20°C in small aliquots. Proteasome inhibitor MG132 …
Web0.5 mg/mL stock solution - autoclave 200 mL water then add 100 mg Cycloheximide powder and mix After autoclaving agar, add 10 mg/L of stock solution, so 500 ml agar, add 10 mL of 0.5 mg/mL stock ... WebMar 14, 2024 · Add 2 mL of cycloheximide stock solution. Dispense 6 mL of hot medium into each of 32 capped test tubes and sterilize at 250 °F (121 °C) for 10 min. Store in the lab refrigerator. To prepare HLP, sterilize 13 screw-cap tubes (with caps partially screwed on) and a 125-mL beaker covered with aluminum foil for 15 min at 250 °F (121 °C).
WebHow to make cycloheximide stock? Asked 3rd Mar, 2024 Caroline Marden I need to use an anti fungal and have got cycloheximide, is this right? 0.5 mg/mL stock solution - autoclave 200 mL water... WebApr 13, 2024 · The frozen stock was inoculated into TCM1 Glc− as described below. TCM1 is a synthetic medium based on CDM15 medium 20 (for T. thermophila ) and BG-11 medium 24 (for Synechocystis sp. PCC6803).
WebJun 17, 2024 · Cycloheximide Stock Solution - Weigh 5 gr of cycloheximide - Measure 100 ml of acetone - Mix Sabouraud dextrose agar medium (Emmons) is prepared as previously described and additionally for each liter of medium add 10 ml of cycloheximide stock solution before autoclaving. Sabouraud dextrose agar (Emmons) with …
WebCycloheximide solution. Synonyms: CHX. Empirical Formula (Hill Notation): C15H23NO4. CAS Number: 66-81-9. Molecular Weight: 281.35. conley perryWebCycloheximide (NSC-185, Actidione, Naramycin A, CHX, FT 3422-2, NM-MCD 80), an antifungal antibiotic, is an eukaryote protein synthesis inhibitor with IC50 of 532.5 nM … edgewood to albuquerqueWebMay 4, 2024 · Cycloheximide (stock solution 5 mM in water; use at a 1:1000 dilution). 9. Sterile glass coverslips (22 × 22 mm) placed in sterile 35 mm plastic tissue culture dishes. 2.2 Photosensitizing Agents and Irradiation 1. Benzoporphyrin derivative (BPD; VWR) is dissolved in DMSO at a 1 mM concentration. Incubate cells with a 0.5 μM concentration … conley paulWebCycloheximide (microbial) ≥94%, white powder Supplier: MP Biomedicals Description: Applications Cycloheximide is used in protein synthesis in apoptosis. It is also used in plant research to study disease resistance and as... SDS Cycloheximide 95% Supplier: Thermo Scientific Chemicals Description: CYCLOHEXIMIDE 1G New Product edgewood town center giant eagleWebPrice $31.00 - $211.00 Detailed Description Cycloheximide Solution (10% in DMSO, Sterile) is equivalent to 100 mg/ml. It is a glutarimide antibiotic and natural fungicide isolated from Streptomyces griseus and a protein synthesis inhibitor in eukaryotic cells. It was discovered by Alma Whiffen-Barksdale of Upjohn Company in 1946. edgewood top grain leather sofaWebCycloheximide solution Synonyms: CHX Empirical Formula (Hill Notation): C15H23NO4 CAS Number: 66-81-9 Molecular Weight: 281.35 Product Comparison Guide Use the product attributes below to configure the comparison table. (Select up to 3 total.) Select Attribute Select Attribute Select Attribute Sort by: Default Product Number Product … edgewood town center storesWebCycloheximide is an anti-fungal agent used in preparing microbiological culture media in a laboratory setting. Cycloheximide is not intended for use in the diagnosis of disease or … conley pdc